Structures of translationally inactive mammalian ribosomes
نویسندگان
چکیده
منابع مشابه
Characterizing inactive ribosomes in translational profiling.
The broad impact of translational regulation has emerged explosively in the last few years in part due to the technological advance in genome-wide interrogation of gene expression. During mRNA translation, the majority of actively translating ribosomes exist as polysomes in cells with multiple ribosomes loaded on a single transcript. The importance of the monosome, however, has been less apprec...
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QSR1 is a highly conserved gene which encodes a 60S ribosomal subunit protein that is required for joining of large and small ribosomal subunits. In this report we demonstrate heterologous complementation of a yeast QSR1 deletion strain with both the human and corn homologs and show that the human and corn proteins are assembled into hybrid yeast/human and yeast/corn ribosomes. While the homolo...
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The puromycin-induced dissociation, at high ionic strength, of active ribosomes from reticulocytes resulted in the release of protein-free, apparently undegraded, messenger RNA for globin, which was identified by centrifugation on a sucrose density gradient. This procedure should make messenger RNA from various cells available for isolation on a large scale, and has several advantages over proc...
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X-chromosome inactivation (XCI), the random transcriptional silencing of one X chromosome in somatic cells of female mammals, is a mechanism that ensures equal expression of X-linked genes in both sexes. XCI is initiated in cis by the noncoding Xist RNA, which coats the inactive X chromosome (Xi) from which it is produced. However, trans-acting factors that mediate XCI remain largely unknown. H...
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Following translation termination, ribosomal subunits dissociate to become available for subsequent rounds of protein synthesis. In many translation-inhibiting stress conditions, e.g. glucose starvation in yeast, free ribosomal subunits reassociate to form a large pool of non-translating 80S ribosomes stabilized by the 'clamping' Stm1 factor. The subunits of these inactive ribosomes need to be ...
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ژورنال
عنوان ژورنال: eLife
سال: 2018
ISSN: 2050-084X
DOI: 10.7554/elife.40486